Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthetic Biology02:55

Synthetic Biology

4.4K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.4K
Types of RNA01:23

Types of RNA

61.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
61.3K
Bioreactor Controls-III01:22

Bioreactor Controls-III

67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67
Translational Regulation01:29

Translational Regulation

877
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
877
Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
Riboswitches01:56

Riboswitches

8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A CRISPR-Cas13d cancer therapeutic enables selective elimination of uveal melanoma.

Molecular therapy. Oncology·2026
Same author

CRISPR-Cas-based live cell imaging of genome dynamics.

Nature reviews. Genetics·2026
Same author

The WalRK two-component system in <i>Streptococcus pneumoniae</i> ensures robustness of secondary wall polymer attachment.

bioRxiv : the preprint server for biology·2026
Same author

Programmable macromolecule delivery via engineered trogocytosis.

Nature cell biology·2026
Same author

Rewriting the epigenome: CRISPR tools for biological discovery and therapeutics.

Current opinion in biomedical engineering·2026
Same author

Origin of replication discovery for environmentally isolated <i>Pantoea</i> strain enables expression of heterologous proteins, pathways and products.

iScience·2026
Same journal

Reframing risk assessment for malaria elimination in a changing climate.

Nature reviews. Microbiology·2026
Same journal

Bacterial vesicles protect the membrane during polymyxin stress.

Nature reviews. Microbiology·2026
Same journal

Fermented food microbiome: influence on oral and gut microbiota, and human health.

Nature reviews. Microbiology·2026
Same journal

Klebsiella genus as driver of human disease: from infections to non-communicable disorders.

Nature reviews. Microbiology·2026
Same journal

Coupling experiments and macroecological models to resolve multi-stressor effects in vector-pathogen systems.

Nature reviews. Microbiology·2026
Same journal

A new antibiotic scaffold hits a new target.

Nature reviews. Microbiology·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

11.9K

A versatile framework for microbial engineering using synthetic non-coding RNAs.

Lei S Qi1, Adam P Arkin2

  • 11] University of California San Francisco Center for Systems and Synthetic Biology, University of California San Francisco, San Francisco, California 94158, USA. [2] Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94158, USA. [3] California Institute for Quantitative Biomedical Research, San Francisco, California 94158, USA.

Nature Reviews. Microbiology
|April 17, 2014
PubMed
Summary
This summary is machine-generated.

Engineered synthetic non-coding RNAs offer programmable functions for signal sensing and gene regulation. These RNA devices are advancing genetic and cellular engineering across diverse microorganisms.

More Related Videos

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

14.2K
Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

2.1K

Related Experiment Videos

Last Updated: May 1, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

11.9K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

14.2K
Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

2.1K

Area of Science:

  • Synthetic biology
  • Molecular biology
  • RNA engineering

Background:

  • Synthetic non-coding RNAs are versatile molecular devices with programmable functions.
  • Their structure and function rely on Watson-Crick base pairing.
  • These RNA regulators operate at DNA, mRNA, and protein levels.

Purpose of the Study:

  • To review recent advancements in engineering synthetic non-coding RNA devices.
  • To highlight applications of these RNA devices in genetic and cellular engineering.
  • To cover their use in a wide range of microorganisms.

Main Methods:

  • Experimental characterization of synthetic non-coding RNA regulators.
  • Computational modeling of RNA structure, function, and interactions.
  • Integration of engineered RNA devices into genetic circuits.

Main Results:

  • Development of distinct types of synthetic non-coding RNA regulators.
  • Demonstration of programmable functions including signal sensing and gene regulation.
  • Successful application in creating complex synthetic biological systems.

Conclusions:

  • Synthetic non-coding RNA engineering is a rapidly progressing field.
  • These engineered RNAs are powerful tools for genetic and cellular engineering.
  • Applications span diverse microbial systems, enabling efficient synthetic biology.