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Related Experiment Video

Updated: May 1, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
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Paternity identification in sugarcane polycrosses by using microsatellite markers.

M A Xavier1, L R Pinto2, T M Fávero1

  • 1Centro Avançado da Pesquisa Tecnológica do Agronegócio de Cana - IAC/APTA, Ribeirão Preto, SP, Brasil.

Genetics and Molecular Research : GMR
|April 17, 2014
PubMed
Summary
This summary is machine-generated.

Identifying the male parent in sugarcane polycrosses is challenging. Microsatellite markers successfully identified the male parent in 73% of elite clones, improving sugarcane breeding accuracy.

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Area of Science:

  • Plant Breeding
  • Molecular Genetics
  • Agricultural Science

Background:

  • Polycrosses are vital for assessing sugarcane cross-combinations but leave the male parent of progenies unidentified.
  • Accurate parentage is crucial for effective sugarcane breeding programs and selecting high-performance clones.

Purpose of the Study:

  • To integrate molecular marker technology with sugarcane polycrosses.
  • To identify the unknown male parent of elite half-sib sugarcane progenies using microsatellite markers.

Main Methods:

  • Applied 10 microsatellite (single sequence repeats - SSRs) primer pairs to 41 elite sugarcane clones from polycross families.
  • Identified the male parent by analyzing SSR markers present in the clone but absent in the female parent.
  • Calculated average genetic similarity among parents (45.9%) and analyzed allele numbers per SSR.

Main Results:

  • Successfully identified the most likely male parent for 73% of the evaluated sugarcane clones.
  • Detected probable instances of contamination within the polycross families.
  • Microsatellite analysis provided a high number of alleles per SSR, indicating marker efficacy.

Conclusions:

  • Molecular marker technology, specifically SSRs, is essential for identifying and confirming male parents in sugarcane polycross breeding.
  • This approach enhances the accuracy and efficiency of selecting superior sugarcane clones for breeding programs.