Related Experiment Video
Updated: May 1, 2026
08:01
A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
Published on: August 29, 2020
1.9K
Cellular extract facilitates nuclear reprogramming by altering DNA methylation and pluripotency gene expression.
Xian-Rong Xiong1, Dao-Liang Lan, Jian Li
11 College of Life Science and Technology, Southwest University for Nationalities , Chengdu, Sichuan, 610041, China .
Cellular Reprogramming
|April 18, 2014
Summary
Adipose-derived stem cell extracts promote yak fibroblast reprogramming, enhancing pluripotency and improving cloned embryo development. This method offers a novel, non-genetic approach for somatic cell dedifferentiation.
Area of Science:
- Cell biology
- Epigenetics
- Regenerative medicine
Background:
- Differentiated cells maintain identity via epigenetic modifications.
- Cellular reprogramming to pluripotency has applications in disease and regeneration.
Purpose of the Study:
- To investigate the reprogramming of yak fibroblast cells using mouse adipose-derived stem cell (ADSC) extracts.
- To understand the epigenetic changes associated with this reprogramming process.
Main Methods:
- Yak fibroblast cells were permeabilized and incubated with ADSC extracts.
- Analyzed colony formation, pluripotent gene expression, histone modifications, and DNA methylation.
- Assessed the developmental rates of cloned embryos derived from treated cells.
Main Results:
- ADSC extract treatment enhanced colony formation and pluripotent gene expression.
- Observed loss of repressive histone marks and global demethylation.
- Decreased expression and increased methylation of differentiation genes (Col1a1, Col1a2) were noted.
- Significantly improved eight-cell and blastocyst formation rates in cloned embryos.
Conclusions:
- ADSC extract treatment modifies nuclear reprogramming in yak fibroblast cells.
- This study presents a non-genetic methodology for somatic cell dedifferentiation and reprogramming.
- The findings suggest a potential for efficient reprogramming without genetic alteration.
More Related Videos
07:08Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
Published on: February 2, 2024
1.7K
11:53An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
Published on: June 1, 2018
6.1K
Related Concept Videos
01:24Methods of Nuclear Reprogramming
1.4K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.4K
01:29Somatic to iPS Cell Reprogramming
2.1K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.1K
01:14Introduction to Nuclear Reprogramming
1.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
1.3K
01:32Chromatin Modification in iPS Cells
1.5K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.5K
01:14Maintenance of the ES Cell State
1.9K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
1.9K
01:37Epigenetic Regulation
3.5K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.5K