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Optimal flagging combinations for best performance of five blood cell analyzers
M Depoorter1, S Goletti, D Latinne
1Cliniques Universitaires Saint-Luc, Brussels, Belgium.
International Journal of Laboratory Hematology
|April 18, 2014
Summary
Hematology analyzers show variable flagging performance for abnormal cells. Optimizing flag combinations and thresholds is crucial for accurate detection and selecting the best analyzer for specific needs.
Area of Science:
- Hematology
- Clinical Pathology
- Laboratory Automation
Background:
- Core laboratory automation requires efficient hematology analyzers and algorithms for abnormal sample detection.
- Assessing flagging strategies is essential for improving diagnostic accuracy in hematology.
Purpose of the Study:
- To evaluate the impact of combining flags or adjusting trigger levels on identifying abnormal samples.
- To compare the flagging performance of different hematology analyzers for abnormal cell detection.
Main Methods:
- Analysis of 517 EDTA samples from patients with hematological malignancies.
- Utilized Sysmex XE2100/XN2000, Abbott Cell-Dyn Sapphire, Beckman Coulter DXH800, and Siemens ADVIA 2120 analyzers.
- Performed blood smear and buffy coat analysis for each sample.
Main Results:
- Analyzer performance in detecting abnormal cells varied significantly based on flagging strategies and thresholds.
- Siemens ADVIA 2120 and Sysmex XN2000 demonstrated excellent performance in blast detection.
- Beckman Coulter DXH800 showed high sensitivity for abnormal lymphocytes, while Sysmex XN2000 excelled in identifying immature granulocytes and nucleated red blood cells.
Conclusions:
- Flagging performance is inconsistent across different hematology analyzer manufacturers.
- This study provides a baseline for laboratory professionals to select appropriate analyzers and flagging schemes.
