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Easy washing of lysed cell plugs for bacterial typing by pulsed-field gel electrophoresis using simple equipment
Koichi Murakami1, Tamie Noda2, Eriko Maeda1
1Department of Health Science, Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan.
Journal of Microbiological Methods
|April 18, 2014
Abstract:
We designed and tested equipment to wash plugs following cell lysis in pulsed-field gel electrophoresis (PFGE). Our system can wash 30 plugs simultaneously in 1h using 15L of Tris-EDTA buffer, which makes plug washing for PFGE less labor-intensive.

