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qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
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Twenty years of qPCR: a mature technology?
Alessandro Raso1, Roberto Biassoni
1Laboratorio U.O.C. Neurochirurgia, Istituto Giannina Gaslini, Genova, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|April 18, 2014
Abstract:
Quantitative PCR is the "gold standard" technology to quantify nucleic acids and, since the first report describing real-time PCR detection in 1993, its use has been grown exponentially. More recent technological advancements have extended the field of applications ranging from high-resolution melting detection to digital PCR. Nowadays, it is a very accessible technique, but some pitfalls should be overcome in order to achieve robust and reliable analysis.
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