Related Experiment Video
Updated: May 1, 2026
Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
Published on: June 6, 2025
Real-time quantification assay to monitor BCR-ABL1 transcripts in chronic myeloid leukemia
Pierre Foskett1, Gareth Gerrard, Letizia Foroni
1Imperial Molecular Pathology Laboratory, Imperial College London, G Block 2nd floor, North Corridor, Hammersmith Hospital Campus, Du Cane Road, London, W12 OHS, UK, pierre.foskett@imperial.nhs.uk.
Abstract:
The BCR-ABL1 fusion gene, the causative lesion of chronic myeloid leukemia (CML) in >95 % of newly presenting patients, offers both a therapeutic and diagnostic target. Reverse-transcription quantitative polymerase chain reaction technology (RT-qPCR), utilizing primer-probe combinations directed to exons flanking the breakpoint junctional region, offers very high levels of both specificity and sensitivity, in a scalable, robust, and cost-effective assay.
More Related Videos
09:57Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
Published on: March 5, 2018
08:46A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
Published on: November 12, 2019