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In vitro pemphigus vulgaris model using organotypic cultures of human epidermal keratinocytes
T Hunziker1, C Boillat, H A Gerber
1Department of Dermatology, University of Berne, Switzerland.
The Journal of Investigative Dermatology
|August 1, 1989
Abstract:
Using the Combi-ring-dish (CRD), a new culture device, organotypic cultures of human epidermal keratinocytes were grown on bovine eye lens capsules. In these highly differentiated cultures, typical suprabasal acantholysis was induced by pemphigus vulgaris antibodies. This in vitro pemphigus vulgaris model may be used to analyse keratinocyte-derived factors causing acantholysis in experimental pemphigus.