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ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
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Multiplex lateral flow immunoassay for mycotoxin determination.

Suquan Song1, Na Liu, Zhiyong Zhao

  • 1Institute for Agro-food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences , 1000 Jinqi Road, Shanghai 201403, China.

Analytical Chemistry
|April 22, 2014
PubMed
Summary
This summary is machine-generated.

A new multiplex lateral flow immunoassay (LFA) accurately detects aflatoxin B1 (AFB1), zearalenone (ZEA), and deoxynivalenol (DON) in cereals. This rapid test meets EU limits and shows good agreement with LC-MS/MS, offering a reliable screening tool.

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Area of Science:

  • Food safety and analytical chemistry
  • Development of rapid immunoassay techniques
  • Mycotoxin detection in agricultural commodities

Background:

  • Mycotoxins like aflatoxin B1 (AFB1), zearalenone (ZEA), and deoxynivalenol (DON) pose significant risks to food safety.
  • Existing detection methods can be time-consuming and require specialized equipment.
  • There is a need for rapid, sensitive, and cost-effective methods for mycotoxin analysis in cereals.

Purpose of the Study:

  • To develop and validate a novel multiplex lateral flow immunoassay (LFA) for the simultaneous detection of AFB1, ZEA, and DON in cereal samples.
  • To assess the sensitivity, specificity, and accuracy of the developed LFA compared to established methods.
  • To determine if the LFA meets regulatory requirements, such as European Union (EU) maximum levels.

Main Methods:

  • Development of a class-specific antibody-based multiplex lateral flow immunoassay.
  • Determination of visual and calculated limits of detection (vLOD and cLOD), and cutoff values for each mycotoxin.
  • Evaluation of assay performance using spiked samples, including recovery and relative standard deviation (RSD) assessments.
  • Validation against liquid chromatography-tandem mass spectrometry (LC-MS/MS) using naturally contaminated maize samples.

Main Results:

  • The LFA demonstrated visual LODs of 0.03 μg/kg for AFB1, 1.6 μg/kg for ZEA, and 10 μg/kg for DON.
  • Calculated LODs were 0.05 μg/kg (AFB1), 1 μg/kg (ZEA), and 3 μg/kg (DON), all below EU maximum levels.
  • Recoveries ranged from 80% to 122% with RSDs between 5% and 20%.
  • The LFA showed good agreement with LC-MS/MS (100% for DONs and AFs, 81% for ZEAs), though a general overestimation was noted.

Conclusions:

  • The developed multiplex LFA is a sensitive and specific tool for the qualitative and semi-quantitative determination of AFB1, ZEA, and DON in cereals.
  • The assay's performance characteristics, including low detection limits, make it suitable for routine screening and compliance with regulatory standards.
  • While exhibiting good agreement with LC-MS/MS, users should be aware of potential overestimation in quantification compared to chromatographic methods.