Search research articles

Related Concept Videos

Silica Gel Column Chromatography: Overview

Silica Gel Column Chromatography: Overview

Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A green solvent-free method for methylene blue quantification in water by dSPE-DRIFTS using MCM-41 mesoporous silica.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026

Same author

A synthetic microbial community for soybean biofertilization designed via chlorophyll-based iterative selection.

Applied and environmental microbiology·2026

Same author

Modulation optimization when using a splitter pump after the first dimension in comprehensive two- dimensional liquid chromatography.

Journal of chromatography. A·2024

Same author

Hybrid organic monolithic column containing MIL-68(Al) for the separation of small molecules by capillary HPLC.

Journal of chromatography. A·2024

Same author

Enantioseparation of pesticides by gas chromatography. Measurement of association constants enantiomer-chiral selector.

Journal of chromatography. A·2024

Same author

Optimization of resolution as a simultaneous function of solvent composition, pH and temperature in reversed-phase liquid chromatography.

Analytica chimica acta·2024

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 1, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

New method for sintering silica frits for capillary microcolumns.

Sonia Keunchkarian¹, Pablo J Lebed¹, Brenda B Sliz¹

¹Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA), Universidad Nacional de La Plata, Calle 47 y 115 (1900), La Plata, Buenos Aires, Argentina.

Analytica Chimica Acta

|April 22, 2014

Summary

This study introduces a novel method for creating robust silica frits for chromatography columns. The new technique ensures better frit quality, improved column efficiency, and avoids bubble formation.

Keywords:

Capillary microcolumns Electrochromatography Sintered silica frits

More Related Videos

Author Spotlight: Standardizing the Development of Amine-Based Silica Composites as CO2 Adsorbents for Direct Air Capture

Author Spotlight: Standardizing the Development of Amine-Based Silica Composites as CO2 Adsorbents for Direct Air Capture

Published on: September 29, 2023

$Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing$

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Related Experiment Videos

Last Updated: May 1, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

Author Spotlight: Standardizing the Development of Amine-Based Silica Composites as CO2 Adsorbents for Direct Air Capture

Author Spotlight: Standardizing the Development of Amine-Based Silica Composites as CO2 Adsorbents for Direct Air Capture

Published on: September 29, 2023

$Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing$

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Area of Science:

Analytical Chemistry
Materials Science

Background:

Manufacturing robust packed capillary microcolumns requires effective porous retaining devices (frits).
Conventional frit production methods using radial heating of sintered silica particles have drawbacks.
Silica particles are ideal for frits due to mechanical resistance and efficiency.

Purpose of the Study:

To develop an improved method for producing silica-based retaining devices for packed capillary microcolumns.
To overcome the limitations of conventional radial heating methods for frit fabrication.

Main Methods:

Investigated frontal heat exposure of a packed silica particle bed at the capillary tube end.
Utilized radial insulation and axial heat transfer from a muffle oven.
Focused on generating short, highly permeable frits.

Main Results:

Achieved improved radial homogeneity in frit formation.
Protected the external polyimide coating during the heating process.
Generated extremely short frits (400-600 μm) with high permeability.
Successfully avoided bubble formation within the frits.

Conclusions:

The new frontal heating method offers substantial advantages over conventional radial heating for frit production.
This technique yields superior silica frits, enhancing packed capillary microcolumn performance.
The method is suitable for manufacturing high-efficiency, bubble-free chromatography columns.