A mouse monoclonal antibody against Alexa Fluor 647
Irene Wuethrich1, Eduardo Guillen, Hidde L Ploegh
1Whitehead Institute for Biomedical Research , Cambridge, Massachusetts.
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
|April 22, 2014
Abstract:
Fluorophores are essential tools in molecular and cell biology. However, their application is mostly confined to the singular exploitation of their fluorescent properties. To enhance the versatility and expand the use of the fluorophore Alexa Fluor 647 (AF647), we generated a mouse monoclonal antibody against it. We demonstrate its use of AF647 for immunoblot, immunoprecipitation, and cytofluorimetry.
