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Production and characterization of monoclonal antibodies specific for Mycobacterium bovis
P R Wood1, J Ripper, A J Radford
1CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.
Abstract:
A series of monoclonal antibodies (MAbs), specific for Mycobacterium bovis and BCG strains, were tested extensively for cross-reactivity to a wide range of mycobacterial species using ELISA, Western blotting and dot-blot analysis. The MAbs bound specifically to M. bovis and BCG and showed limited cross-reactivity with some strains of M. tuberculosis. All these MAbs recognized a 22 kDa protein previously termed MPB70, and by competitive ELISA analysis appeared to detect at least three M. bovis-specific determinants on the MPB70 molecule.
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