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Using a Bipolar Electrode to Create a Temporal Lobe Epilepsy Mouse Model by Electrical Kindling of the Amygdala
Published on: June 29, 2022
The influence of neuropathology on brain inflammation in human and experimental temporal lobe epilepsy
M W Aalbers1, K Rijkers2, H J M Majoie3
1School for Mental Health & Neuroscience, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands; Department of Neurology, Maastricht University Medical Centre, PO Box 5800, 6202 AZ Maastricht, The Netherlands.
Abstract:
It is unclear to what extent neuropathological changes contribute to brain inflammation observed in temporal lobe epilepsy (TLE). Here, we compared cytokine levels between histopathologically-confirmed sclerotic hippocampi and histopathologically-confirmed normal hippocampi from TLE patients. We analyzed a similar cytokine panel in the hippocampi of amygdala-kindled rats and we evaluated neuropathological changes by immunohistochemistry. In TLE patients, cytokine levels were not significantly different between sclerotic and non-sclerotic hippocampi. Though kindling resulted in increased astrocyte activation, cytokine levels and microglia activation were unchanged. These results suggest that the chronic epileptic state in TLE can also occur in the absence of intracerebral inflammation. Highlights.
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