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Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Introduction to Nuclear Reprogramming01:14

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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Reproductive Cloning01:27

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Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
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Somatic to iPS Cell Reprogramming01:29

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Updated: May 1, 2026

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
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Human somatic cell nuclear transfer using adult cells.

Young Gie Chung1, Jin Hee Eum2, Jeoung Eun Lee2

  • 1Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, CA 90036, USA; CHA Stem Cell Institute, CHA University, Seoul 135-081, Korea.

Cell Stem Cell
|April 22, 2014
PubMed
Summary

Patient-specific human pluripotent stem cells were generated from adult skin cells using somatic cell nuclear transfer (SCNT). This breakthrough demonstrates SCNT

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Area of Science:

  • Stem cell biology
  • Reproductive medicine
  • Genetics

Background:

  • Somatic cell nuclear transfer (SCNT) can create patient-specific pluripotent stem cells for regenerative medicine.
  • Previous human SCNT success was limited to fetal or infant cells.

Purpose of the Study:

  • To investigate the feasibility of generating human embryonic stem cells (ESCs) from adult somatic cells using SCNT.
  • To demonstrate SCNT's applicability with aged human cells.

Main Methods:

  • Generation of human ESCs using SCNT with dermal fibroblasts from adult males (35 and 75 years old).
  • Utilized a recently developed SCNT methodology.

Main Results:

  • Successfully derived patient-specific human ESCs from adult dermal fibroblasts.
  • Demonstrated SCNT's effectiveness with aged somatic cells.

Conclusions:

  • SCNT is applicable for generating patient-specific stem cells from adult human cells.
  • Supports SCNT as a viable strategy for regenerative medicine applications.