Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Could NAPQI Contribute to Paracetamol Analgesia? Redox Modulation of Kv7 Ion Channels.

Pharmacology research & perspectives·2026
Same author

Role of natural inter-individual variability in the different penetrance of congenital long QT syndromes (LQTS) types 1 and 2: an in silico approach.

Scientific reports·2026
Same author

Calmodulin assists during co-translational folding of the K<sub>V</sub>7.2 channel calcium responsive domain.

Protein science : a publication of the Protein Society·2026
Same author

Editorial: Ion channels in the nervous system.

Frontiers in cellular neuroscience·2026
Same author

Proteomics Reveals Differential Diagnosis Biomarkers Between Sepsis and Hemophagocytic Syndrome.

Biomedicines·2025
Same author

Thyrotropin Modulates Calcium Handling and Contractility in Adult Cardiac Myocytes.

Heart, lung & circulation·2025

Related Experiment Video

Updated: May 1, 2026

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
08:55

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

Published on: April 25, 2018

8.4K

Ionic channels underlying the ventricular action potential in zebrafish embryo.

Aintzane Alday1, Hiart Alonso2, Monica Gallego2

  • 1Department of Physiology, School of Pharmacy, University of the Basque Country UPV/EHU, Spain.

Pharmacological Research
|April 22, 2014
PubMed
Summary

Zebrafish embryos

Keywords:
Cardiac developmentDepolarizing currentElectrophysiologyRepolarizing current

More Related Videos

Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
07:04

Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

Published on: February 9, 2022

2.2K
Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos
07:29

Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos

Published on: August 13, 2021

5.6K

Related Experiment Videos

Last Updated: May 1, 2026

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
08:55

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

Published on: April 25, 2018

8.4K
Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies
07:04

Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

Published on: February 9, 2022

2.2K
Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos
07:29

Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos

Published on: August 13, 2021

5.6K

Area of Science:

  • Cardiovascular Physiology
  • Zebrafish as a Model Organism

Background:

  • Zebrafish embryos are increasingly used in cardiac research due to ethical regulations and their unique characteristics.
  • Direct electrophysiological characterization of zebrafish embryonic cardiac action potentials and ionic currents is limited.

Purpose of the Study:

  • To identify the ionic channels and their roles in zebrafish embryonic ventricular action potentials.
  • To optimize the use of zebrafish embryos as a model for cardiac studies and drug testing.

Main Methods:

  • Electrophysiology (direct recordings of action potentials and ionic currents)
  • Pharmacological blockade of specific ion channels
  • Immunofluorescence to confirm channel protein expression

Main Results:

  • Action potential upstroke relies on Sodium (Na+) and T-type Calcium (Ca+2) channels.
  • Plateau phase is dependent on L-type Ca+2 channels.
  • Repolarization and diastolic potential are mediated by ERG K+ channels.
  • Nav1.5, Cav1.2, Cav3.2, and ERG channel proteins are present; Ito and IKs currents are absent.

Conclusions:

  • This study elucidates the specific ionic mechanisms underlying zebrafish embryonic cardiac action potentials.
  • The findings support the utility of zebrafish embryos for studying human cardiac diseases and for pharmaceutical drug screening.