Related Experiment Video
Updated: May 1, 2026

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
Published on: April 16, 2017
Photothermal microscopy: optical detection of small absorbers in scattering environments
Pierre Vermeulen1, Laurent Cognet, Brahim Lounis
1University of Bordeaux, LP2N, F-33405 Talence, France; Institut d'Optique & CNRS, LP2N, F-33405 Talence, France.
Abstract:
Photothermal microscopy enables detection of nanometer-sized objects solely based on their absorption. This technique allows efficient observation of various nano-objects in scattering media notably gold nanoparticles in cells. The extreme sensitivity of the method and the stability of the signals open numerous applications in spectroscopy, analytical chemistry and bioimaging. This review briefly describes the principle and the main characteristics of photothermal microscopy, with its major advantages and limitations, and exposes the principal applications that have been carried out since its first implementation.
Related Concept Videos
Absorption of Radiation
Total Internal Reflection Fluorescence Microscopy
UV–Vis Spectrometers
Photoluminescence: Applications
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Flame Photometry: Overview

