Polysaccharides isolated from Digenea simplex inhibit inflammatory and nociceptive responses

Area of Science:

• Marine natural products
• Pharmacology
• Immunology

Background:

• Polysaccharides (PLS) are known for diverse pharmacological activities.
• The anti-inflammatory and antinociceptive potential of PLS from Digenea simplex remains largely unexplored.

Purpose of the Study:

• To investigate the anti-inflammatory and antinociceptive effects of PLS isolated from the marine red alga Digenea simplex.

Main Methods:

• Carrageenan-induced paw edema model in mice.
• Inflammation inhibition assays using dextran, histamine, serotonin, and bradykinin.
• Neutrophil migration inhibition in mouse paw and peritoneal cavity.
• Cytokine level analysis (IL-1β, TNF-α) in peritoneal fluid.
• Pain behavioral tests: acetic acid-induced writhing, formalin test, and hot plate test.

Main Results:

• PLS fraction demonstrated dose-dependent reduction of carrageenan-induced edema.
• Inhibition of inflammatory mediators (dextran, histamine, serotonin, bradykinin) and neutrophil migration.
• Significant decrease in IL-1β and TNF-α levels in peritoneal fluid.
• Reduced abdominal writhing, licking time, and increased pain latency in behavioral tests.

Conclusions:

• PLS from Digenea simplex possesses notable anti-inflammatory and antinociceptive properties.
• These findings suggest PLS as a potential therapeutic agent for inflammatory conditions and pain management.