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The one-step slow freezing method significantly improves embryo and blastomere survival rates compared to the two-step method. This enhanced cryopreservation technique offers greater efficiency for IVF centers without impacting clinical outcomes.

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Area of Science:

  • Reproductive Science
  • Embryology
  • Cryobiology

Background:

  • Slow freezing is a common method for embryo cryopreservation in IVF.
  • Previous research suggests modified one-step methods can improve survival rates.
  • The efficacy of a standard one-step method using commercial kits without compositional changes is less understood.

Purpose of the Study:

  • To evaluate the efficacy of a one-step slow freezing method using a commercial kit (1.5 M PROH with 0.1 M sucrose) compared to the conventional two-step method.
  • To assess the impact on embryo and blastomere survival, cleavage, and clinical outcomes.

Main Methods:

  • Retrospective analysis of 526 thaw cycles involving cleavage stage embryos.
  • Comparison between embryos cryopreserved using a conventional two-step slow freezing method and a modified one-step method.
  • Evaluation of embryo survival rate, blastomere survival rate, cleavage rate, clinical pregnancy rate, and live birth rate.

Main Results:

  • The one-step method showed a significantly higher embryo survival rate (86.9%) than the two-step method (83.1%; p=0.04).
  • Total blastomere survival rate was also significantly increased with the one-step method (81.0% vs. 76.5%; p<0.001).
  • No statistically significant differences were observed in cleavage rates, clinical pregnancy rates, or live birth rates between the two methods.

Conclusions:

  • The one-step slow freezing method is superior to the conventional two-step method for improving embryo and blastomere survival rates.
  • This method does not negatively affect cleavage rates or clinical outcomes in cryopreserved cleavage stage embryos.
  • The one-step method is suitable for routine application in IVF centers for efficient cryopreservation of cleavage stage embryos.