Related Experiment Video
Updated: May 1, 2026

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
Published on: December 20, 2021
Single vs. two-photon microscopy for label free intrinsic tissue studies in the UV light region
Vitalijs Zubkovs1, Frédéric Jamme, Slavka Kascakova
1DISCO Beamline, Synchrotron SOLEIL, F-91192 Gif sur Yvette, France. matthieu.refregiers@synchrotron-soleil.fr.
Abstract:
Fibrillar distribution in the rat tail tendon and mice liver can be measured using optical methods. Two-photon excitation provides easy assessment of fibrotic collagen types I and II. Single photon deep ultraviolet (DUV) excitation imaging highlights all collagen types without discrimination. Their combination on the same tissue area provides a better overview of collagens in fibrillar diseases.
Related Concept Videos
Two-Dimensional Microscopy in Microbiology
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

