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Updated: May 1, 2026

Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Laminated microfluidic system for small sample protein analysis.

Sara Saedinia¹, Kent L Nastiuk², John J Krolewski²

¹University of California, Irvine, 3317 Engineering Gateway, Irvine, California 92697, USA.

Biomicrofluidics

|April 23, 2014

Summary

This summary is machine-generated.

This study introduces modular microfluidic coupons (microfloupons) for creating custom 3D assay devices. This lamination-based technology enables flexible, integrated microfluidic systems for various applications.

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Last Updated: May 1, 2026

Digital Microfluidics for Automated Proteomic Processing

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Published on: November 6, 2009

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

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Published on: April 16, 2016

Area of Science:

Biotechnology
Microfluidics
Materials Science

Background:

Traditional microfluidic devices often require complex, single-unit designs.
Developing integrated systems for multi-step assays can be challenging and time-consuming.

Purpose of the Study:

To present a novel lamination-based technology for fabricating modular 3D microfluidic devices.
To demonstrate the versatility of "microfloupons" for custom assay design and assembly.

Main Methods:

Development of a lamination technique for stacking microfluidic components.
Design and assembly of a proof-of-concept device using microfloupons for protein electrophoresis.
Characterization of the assembled microfluidic device's performance.

Main Results:

Successful demonstration of a 3D microfluidic device for sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
The device enabled precise sample manipulation, injection, electrophoretic separation, and gel recovery.
Microfloupons proved robust, easy to handle, align, and laminate for diverse assay configurations.

Conclusions:

The microfloupon approach offers a convenient and flexible method for constructing integrated microfluidic assay devices.
This modular system simplifies the design of complex, multi-step assays, offering benefits in automation and speed.
The technology supports assays using various materials and allows for pre-manufacturing of components by different entities.