Exploratory study of oral mucosal colonization of human gastric Helicobacter pylori in mice

Area of Science:

• Microbiology
• Oral Biology
• Infectious Diseases

Background:

• Helicobacter pylori (Hp) is a significant human pathogen, primarily associated with gastric infections.
• The role of oral colonization by Hp and its potential contribution to gastric infection remain areas of active investigation.
• Establishing reliable animal models is crucial for understanding Hp's oral-gastric transmission dynamics.

Purpose of the Study:

• To develop a novel mouse model for inducing and studying Helicobacter pylori (Hp) colonization on the buccal mucosa.
• To investigate the feasibility of using artificial oral films for Hp adhesion and biofilm formation in vivo.
• To provide a foundation for future research on the interplay between oral and gastric Hp infections.

Main Methods:

• Human gastric Helicobacter pylori (Hp) was applied to pre-treated mouse buccal mucosa using artificial oral films.
• Sixty BALB/c mice were divided into three groups: Hp film, Hp smear, and a control (black film).
• Hp colonization was assessed using optical and electron microscopy to detect biofilm formation.

Main Results:

• Hp biofilm colonization was successfully detected in mice treated with the Hp artificial oral film.
• Microscopic analysis confirmed Hp accumulation and biofilm development on the buccal mucosa in the Hp film group.
• No Hp biofilms were observed in the Hp smear or control groups, indicating the efficacy of the artificial film method.

Conclusions:

• A workable mouse model for oral Helicobacter pylori (Hp) infection has been established, demonstrating Hp colonization on the buccal mucosa.
• This model provides crucial animal experimental evidence for Hp's growth and colonization in the oral cavity.
• The developed method facilitates further research into dual oral-gastric Hp infections and the relationship between oral and gastric Hp reservoirs.