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Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition
Zhang-Hua Li1, Wen Liao2, Qiang Zhao3
1Department of Orthopedics, Renmin Hospital of Wuhan University Wuhan 430060, China.
Objective:
To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia.
Methods:
The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR.
Results:
After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P<0.05), especial the expression of Cbfa1 (P<0.05).
Conclusion:
After transfected with Cbfa1, cell morphology or growth cycle of MSCs was not significantly affected, but its osteogenic differentiation potential enhanced, particularly its osteogenic differentiation potential under hypoxia enhanced.
