Characterization of white Mahlab (Prunus mahaleb L.) seed oil: a rich source of α-eleostearic acid
Related Experiment Video
Updated: Apr 30, 2026

Author Spotlight: A Tailor-Made Sample Preparation Approach for Enhanced MALDI-IMS Analysis of Hard Palm Seeds
Published on: June 30, 2023
Hassen Mohamed Sbihi1, Imededdine Arbi Nehdi, Saud Ibrahim Al-Resayes
1King Saud Univ., College of Science, Chemistry Dept., P.O. BOX 2454, Riyadh 1145, Saudi Arabia.
Unlabelled:
Seed oils with high polyunsaturated fatty acid content are used in various industries including the food and pharmaceutical industries. White mahlab (Prunus mahaleb L.) seed was found to contain 31% oil. The oil was highly polyunsaturated and abundant in α-eleostearic (38.32%), oleic (31.29%), and linoleic (22.96%) acids, which together comprised 93.91% of the total fatty acids. The α-eleostearic acid was identified and characterized based on (1)H-NMR, UV, and FTIR spectroscopy. The oil was characterized by a relatively high quantity of tocopherols with γ-tocopherol as the major tocopherol isomer. The physicochemical characteristics of the white mahlab seed and seed oil were also determined. The thermogravimetric analysis indicated that the oil was thermally stable up to 350 °C and began to decompose at 520 °C. This study demonstrated that these seeds may be reused and their oil incorporated into other food products, a beneficial practice considering that the compounds present in the seeds and oils have positive effects on human health.
Practical Application:
In this study, mahlab seed oil was found to have potentials to become a new edible oil source as it contained a high level of polyunsaturated fatty acids especially, α-eleostearic acid, which is a conjugated fatty acid rarely found in vegetable oils and has a beneficial effects on human health.
Related Concept Videos
Alkylation of β-Diester Enolates: Malonic Ester Synthesis
Loss of Carboxy Group as CO2: Decarboxylation of Malonic Acid Derivatives
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones
NMR and Mass Spectroscopy of Carboxylic Acids
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the...
Esters to Carboxylic Acids: Saponification
The reaction requires a base in stoichiometric amounts, which participates in the reaction and is not regenerated later. So, the base acts as a...

