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Published on: July 3, 2011
Influence of dilute acid and alkali pretreatment on reducing sugar production from corncobs by crude enzymatic
Rama Raju Baadhe1, Ravichandra Potumarthi2, Naveen Kumar Mekala1
1Department of Biotechnology, National Institute of Technology, Warangal 506004, India.
Abstract:
In the present study, two commonly used catalysts in chemical pretreatment, sulfuric acid and sodium hydroxide, were tested to evaluate the effect of solid-to-liquid ratio on pretreatment and enzymatic hydrolysis. Solid to liquid ratio (S/L) was influential on sugars released with an increase in the S/L ratio between 0.03 and 0.2. Enzymatic digestibility of 0.25 M H2SO4 pretreated corncobs were released more sugars (415.12 mg/mL); whereas, corncobs pretreated with NaOH released 350.12 mg/mL of reducing sugars at S/L 0.05. Further, in comparison with NaOH pretreated corncobs, acid treated material substantially increased the accessibility and digestibility of cellulose during crude enzymatic hydrolysis (28.96 FPU) and released 398.95 mg/mL reducing sugars.
