Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basement membrane and human epidermal differentiation in vitro.

M Guo1, F Grinnell

  • 1Department of Cell Biology and Anatomy, University of Texas Southwestern Medical Center, Dallas 75235.

The Journal of Investigative Dermatology
|September 1, 1989
PubMed
Summary

The basement membrane is not essential for human epidermis differentiation on dermis. However, it is crucial for stable epidermal-dermal adhesion by facilitating hemidesmosome formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial Expression of Concern: Identification of mutations that disrupt phosphorylation-dependent nuclear export of cyclin D1.

Oncogene·2026
Same author

Electrocaloric effects across room temperature in multilayer capacitors.

Nature·2026
Same author

[Transformation and evidence-based progress of chronic constipation treatment mode].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2025
Same author

Natural products in neurodegenerative diseases: recent advances and future outlook.

Frontiers in pharmacology·2025
Same author

[Surgical treatment for slow transit constipation: total colectomy and subtotal colectomy].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2024
Same author

Chromosomal analysis in pregnant women of advanced maternal age: indications for prenatal diagnosis.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2024

Area of Science:

  • Dermatology
  • Tissue Engineering
  • Cell Biology

Background:

  • The basement membrane is a critical component of the dermal-epidermal junction.
  • Its role in the differentiation and adhesion of reconstituted human epidermis is not fully understood.

Purpose of the Study:

  • To investigate the role of the basement membrane in human epidermis differentiation and adhesion on a dermal substrate.
  • To elucidate the structural and functional requirements for stable epidermal-dermal interactions.

Main Methods:

  • Preparation of human dermis with and without basement membrane.
  • Culture of human epidermal cells on these dermal substrata.
  • Immunofluorescence staining for basement membrane components (laminin, type IV collagen).
  • Electron microscopy to assess hemidesmosome formation and ultrastructure.

Related Experiment Videos

  • Assessment of epidermal differentiation markers (keratin synthesis, cell layer development).

    • Main Results:

    • High-level differentiation of reconstituted human epidermis occurred independently of the basement membrane.
    • Epidermal differentiation was evidenced by the formation of basal, spinous, and granular cell layers and keratin synthesis.
    • The basement membrane was essential for regulating adhesive interactions between epidermis and dermis.
    • Epidermal cells formed hemidesmosomes and mechanically stable bonds on dermis with basement membrane.
    • In the absence of the basement membrane, hemidesmosome formation was inhibited, leading to unstable epidermal-dermal bonding.
    • Dermis lacking a basement membrane underwent reorganization by the overlying epidermal cell layer.

    Conclusions:

    • The basement membrane is not required for the differentiation of human epidermis reconstituted on dermis.
    • The basement membrane plays a vital role in establishing mechanically stable adhesion between the epidermis and dermis through hemidesmosome formation.
    • The absence of the basement membrane leads to unstable epidermal-dermal interactions and dermal reorganization.