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Related Concept Videos

Thermosensation01:43

Thermosensation

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Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
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Repressible Operon: trp Operon01:21

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The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
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GPCRs Regulate Adenylyl Cylase Activity01:09

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Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
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G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
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G Protein-coupled Receptors01:15

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G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
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Related Experiment Video

Updated: Apr 30, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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TRPC2.

Barbara A Miller1

  • 1Departments of Pediatrics and Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA, bmiller3@psu.edu.

Handbook of Experimental Pharmacology
|April 24, 2014
PubMed
Summary

Transient Receptor Potential Cation Channel subfamily C member 2 (TRPC2) plays key roles in regulating VNO signaling, red blood cell function, and sperm function. TRPC2 knockout mice exhibit altered behaviors and red blood cell characteristics.

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Physiology

Background:

  • TRPC2 is a pseudogene in humans but functional in rodents.
  • TRPC2 is highly expressed in the Vomeronasal Organ (VNO) and detectable in other tissues like erythroblasts and sperm.
  • Known activators include DAG and erythropoietin; it's inhibited by Ca(2+)-calmodulin.

Purpose of the Study:

  • To investigate the functions of TRPC2 in mammals.
  • To understand the physiological consequences of TRPC2 depletion.

Main Methods:

  • Analysis of TRPC2 knockout mice.
  • Behavioral studies.
  • Hematological analysis of red blood cells.
  • Assessment of calcium influx in sperm and erythroid cells.

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

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Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
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Related Experiment Videos

Last Updated: Apr 30, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
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Main Results:

  • TRPC2 knockout mice showed altered sex discrimination and reduced male-male aggression.
  • Red blood cells in knockout mice had increased MCV, MCH, and hematocrit, with reduced MCHC.
  • TRPC2-depleted red cells exhibited resistance to oxidative stress-induced hemolysis.
  • TRPC2 regulates pheromone signaling, erythropoietin-stimulated calcium influx, and ZP3-evoked sperm calcium influx.

Conclusions:

  • TRPC2 is crucial for pheromone detection in the VNO, impacting social behaviors.
  • TRPC2 plays a significant role in regulating red blood cell homeostasis and stress resistance.
  • TRPC2 is involved in calcium signaling in sperm, potentially affecting fertilization.