Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

2.1K
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
2.1K
Overview of Fatty Acid Metabolism01:28

Overview of Fatty Acid Metabolism

25.4K
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
25.4K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.5K
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

105.4K
Overview
105.4K
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

961
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
961
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

3.2K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic and therapeutic applications of the glycan biomarker H3N2b in GM1 Gangliosidosis.

Molecular genetics and metabolism·2026
Same author

A Non-Classical Neuroactive Steroid Exhibiting Potent, Efficacious GABA<sub>A</sub> Receptor Agonism and NMDA Receptor Inhibition.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: 7-Dehydrocholesterol is an endogenous suppressor of ferroptosis.

Nature·2026
Same author

Structure-activity relationships of steroid and sterol neuromodulators on inflammatory markers in a murine microglial cell line.

The Journal of steroid biochemistry and molecular biology·2026
Same author

Live-Cell Imaging and Lipidomics of Low-Density Lipoprotein Containing Intrinsically Fluorescent Cholesteryl Esters.

Analytical chemistry·2026
Same author

The orientation of cholesterol's hydroxyl group affects its membrane dynamics and intracellular transport.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Apr 30, 2026

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

5.6K

Side-chain oxysterols modulate cholesterol accessibility through membrane remodeling.

Agata A Bielska1, Brett N Olsen, Sarah E Gale

  • 1Diabetic Cardiovascular Disease Center, Washington University School of Medicine , St. Louis, Missouri 63110, United States.

Biochemistry
|April 25, 2014
PubMed
Summary

25-hydroxycholesterol (25-HC) alters cell membrane structure, increasing cholesterol accessibility. This mechanism, driven by membrane thinning, regulates cholesterol homeostasis and cellular transport.

More Related Videos

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
08:45

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

13.2K
Analysis of SCAP N-glycosylation and Trafficking in Human Cells
11:27

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Published on: November 8, 2016

8.2K

Related Experiment Videos

Last Updated: Apr 30, 2026

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

5.6K
LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
08:45

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

13.2K
Analysis of SCAP N-glycosylation and Trafficking in Human Cells
11:27

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Published on: November 8, 2016

8.2K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Dynamics

Background:

  • Side-chain oxysterols, like 25-hydroxycholesterol (25-HC), are crucial for regulating cholesterol homeostasis.
  • Emerging evidence indicates that 25-HC's effects on cholesterol regulation involve alterations to membrane structure.

Purpose of the Study:

  • To investigate how oxysterols affect cholesterol accessibility within cell membranes.
  • To elucidate the role of membrane structural changes in oxysterol-mediated cholesterol regulation.

Main Methods:

  • Utilized perfringolysin O (PFO), a cholesterol-dependent cytolysin, as a biosensor to measure membrane cholesterol accessibility.
  • Employed molecular dynamics simulations to analyze structural changes in the membrane.

Main Results:

  • 25-HC enhances cholesterol accessibility in a lipid composition-dependent manner.
  • Increased cholesterol accessibility correlates with membrane thinning, driven by 25-HC.
  • The 25-HC antagonist agisterol (LY295427) reverses these membrane effects.

Conclusions:

  • Oxysterols modulate the membrane environment, thereby regulating cholesterol accessibility and availability for cellular sensing and transport.
  • Changes in membrane structure are a key mechanism by which oxysterols relay cholesterol regulatory signals.