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Doublecortin may play a role in defining chondrocyte phenotype
Dongxia Ge1, Qing-Song Zhang2, Jovanny Zabaleta3
1Department of Structural and Cellular Biology, Tulane Cancer Center, Louisiana Cancer Research Consortium, Tulane Center for Aging and Tulane Center for Stem Cell Research and Regenerative Medicine, Tulane University Health Sciences Center, New Orleans, LA 70112, USA. gedongx@gmail.com.
International Journal of Molecular Sciences
|April 25, 2014
Summary
Doublecortin (DCX) may influence chondrocyte phenotype. This study used a reporter mouse model to reveal dynamic gene expression during embryonic cartilage development, suggesting DCX
Area of Science:
- Developmental biology
- Cell biology
- Molecular biology
Background:
- Embryonic development of articular cartilage is not well understood.
- The role of doublecortin (DCX) in chondrocyte phenotype determination is unknown.
Purpose of the Study:
- To investigate the role of doublecortin (DCX) in chondrocyte phenotype determination.
- To analyze dynamic gene expression profiles during embryonic handplate development.
Main Methods:
- Utilized a doublecortin (DCX) promoter-driven eGFP reporter mouse model.
- Performed Illumina microarray analysis on mouse embryonic handplates at E12.5-E13.5.
- Expressed human DCX protein in human adipose stem cells and cultured for 14 days.
Main Results:
- Identified differentially expressed genes in distinct regions of the embryonic handplate.
- Revealed unique expression patterns for genes like Cytl1, 3110032G18RIK, Olfr538, Kctd15, Cited1, and Hrc.
- Observed decreased collagen II and increased aggrecan, matrilin 2, and GDF5 upon human DCX expression in stem cells.
Conclusions:
- Doublecortin (DCX) may play a role in defining chondrocyte phenotype.
- Dynamic gene expression patterns in embryonic handplates are region-specific.
- DCX influences extracellular matrix component expression in differentiating cells.
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