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Updated: Apr 30, 2026
A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
Published on: June 15, 2018
Molecular test based on isothermal helicase-dependent amplification for detection of the Clostridium difficile toxin
Catherine Eckert1, Eleonore Holscher2, Amandine Petit3
1Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Antoine, National Reference Laboratory for C. difficile, Paris, France UPMC, Universite Paris 06, GRC-2, Epidiff, Paris, France catherine.eckert@sat.aphp.fr.
Abstract:
The AmpliVue Clostridium difficile assay and a glutamate dehydrogenase (GDH)-illumigene algorithm were evaluated using 308 diarrheal stool specimens of patients suspected of having C. difficile infection. Compared to the enriched toxigenic culture method, the sensitivities, specificities, and positive and negative predictive values of the AmpliVue C. difficile assay and the GDH-illumigene-based algorithm were 91.7% (95% confidence interval [CI], 76.4 to 97.8), 100% (95% CI, 98.3 to 100), 100% (95% CI, 87 to 100), and 98.9% (95% CI, 96.6 to 99.7), respectively.
