Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunogold-silver labeling in histology.

P A In't Veld1

  • 1Department of Pathology, Vrije Universiteit Brussel, Belgium.

The American Journal of Anatomy
|June 1, 1989
PubMed
Summary

Immunogold-silver staining offers high detection efficiency for light-microscopic immunocytochemistry. This technique surpasses traditional peroxidase-based methods in analyzing human biopsy tissues.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reanalysis of study of pancreatic effects of incretin therapy: methodological deficiencies.

Diabetes, obesity & metabolism·2014
Same author

The diagnosis of insulitis in human type 1 diabetes.

Diabetologia·2013
Same author

Insulin crystallization depends on zinc transporter ZnT8 expression, but is not required for normal glucose homeostasis in mice.

Proceedings of the National Academy of Sciences of the United States of America·2009
Same author

Evidence of disturbed meiosis in a man referred for intracytoplasmic sperm injection.

Lancet (London, England)·1998
Same author

Genetic counselling before intracytoplasmic sperm injection.

Lancet (London, England)·1997
Same author

Determination of the parent of origin in nine cases of prenatally detected chromosome aberrations found after intracytoplasmic sperm injection.

Human reproduction (Oxford, England)·1997

Area of Science:

  • Biotechnology
  • Immunocytochemistry
  • Microscopy

Background:

  • Immunogold-silver staining is an emerging technique in immunocytochemistry.
  • It utilizes silver enhancement of gold nanoparticles for signal amplification.
  • This method targets immunologically bound gold probes to antigenic sites in tissue.

Purpose of the Study:

  • To compare the detection efficiency of immunogold-silver staining with the peroxidase-based ABC technique.
  • To evaluate these methods in routinely processed, paraffin-embedded human biopsy samples.

Main Methods:

  • Immunogold-silver staining (IGSS) was employed.
  • Peroxidase-based avidin-biotin complex (ABC) technique was used as a comparator.
  • Both techniques were applied to human biopsy material embedded in paraffin.

Main Results:

  • Immunogold-silver staining demonstrated superior detection efficiency compared to the ABC technique.
  • The study assessed the performance in standard paraffin-embedded human tissues.

Conclusions:

  • Immunogold-silver staining represents a significant advancement in light-microscopic immunocytochemistry.
  • It offers enhanced sensitivity for detecting antigens in human biopsy specimens.

Related Experiment Videos