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Investigation of the stereochemical course of DNA synthesis catalysed by human immunodeficiency virus type 1 reverse
S Hopkins1, P A Furman, G R Painter
1Division of Virology, Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709.
Biochemical and Biophysical Research Communications
|August 30, 1989
Abstract:
The Sp-isomer of thymidine 5'-O-(1-thiotriphosphate) is used as substrate by HIV-1 reverse transcriptase. The absolute configuration of the internucleotide linkage in the oligonucleotide product was identified by 31p NMR spectroscopy to be the Rp-isomer, indicating that incorporation of dTTP alpha S into the oligonucleotide proceeded with inversion of configuration at the alpha-phosphorus. The mechanistic implications of these observations are discussed.