Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interferon-induced early changes in nuclear protein interactions with the interferon consensus sequence.

C Roy1, B Lebleu

  • 1U.A. CNRS 1191, Laboratoire de Biochimie des Protéines, Université de Montpellier II, France.

Biochemical and Biophysical Research Communications
|August 30, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arginine-rich cell penetrating peptides: design, structure-activity, and applications to alter pre-mRNA splicing by steric-block oligonucleotides.

Journal of peptide science : an official publication of the European Peptide Society·2008
Same author

Cell-penetrating-peptide-based delivery of oligonucleotides: an overview.

Biochemical Society transactions·2007
Same author

Cell-penetrating peptide-morpholino conjugates alter pre-mRNA splicing of DMD (Duchenne muscular dystrophy) and inhibit murine coronavirus replication in vivo.

Biochemical Society transactions·2007
Same author

Inhibition of PAI-1 expression in breast cancer carcinoma cells by siRNA at nanomolar range.

Biochimie·2007
Same author

Peptide-based delivery of nucleic acids: design, mechanism of uptake and applications to splice-correcting oligonucleotides.

Biochemical Society transactions·2007
Same author

[GU imaging: an update from RSNA 2002].

Journal de radiologie·2003

Interferons regulate gene transcription through a specific DNA sequence. While interferon treatment alters protein binding to this sequence, the amounts of the identified 114 and 50 kDa proteins remain unchanged.

Area of Science:

  • Molecular Biology
  • Genetics
  • Immunology

Background:

  • Gene transcription is regulated by specific DNA sequences.
  • Interferons (IFN) are key signaling molecules in the immune response.
  • The interferon consensus sequence (ICS) is a regulatory element involved in IFN-mediated gene expression.

Purpose of the Study:

  • To investigate the proteins that bind to the interferon consensus sequence.
  • To determine if interferon treatment affects the binding of nuclear proteins to the ICS.
  • To characterize the molecular weight of proteins interacting with the ICS.

Main Methods:

  • Electrophoretic mobility shift assay (EMSA) using a synthetic 29-mer probe for the ICS.
  • DNase I footprinting to identify protected regions on the DNA.

Related Experiment Videos

  • Nuclear protein blotting (Western blot) to detect specific proteins.

    • Main Results:

    • A synthetic probe for the ICS showed retarded bands with HeLa cell nuclear extracts.
    • Interferon treatment of cells led to an increased amount of the retarded band.
    • DNase I footprinting revealed differential protection of a DNA region upon IFN treatment.
    • Nuclear protein blotting identified two specific proteins of 114 kDa and 50 kDa that bind to the ICS.
    • The amounts of these two proteins were not dependent on IFN treatment.

    Conclusions:

    • Specific nuclear proteins bind to the interferon consensus sequence.
    • Interferon treatment influences the binding activity at the ICS, but not the absolute or relative amounts of the identified 114 and 50 kDa proteins.