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Lectin-tagged fluorescent polymeric nanoparticles for targeting of sialic acid on living cells
Jaebum Cho1, Keiichiro Kushiro, Yuji Teramura
1Department of Bioengineering, Grad10-ate School of Engineering, The University of Tokyo , Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8656, Japan.
Abstract:
In this study, we fabricated lectin-tagged fluorescent polymeric nanoparticles approximately 35 nm in diameter using biocompatible polymers conjugated with lectins for the purpose of detecting sialic acid on a living cell surface, which is one of the most important biomarkers for cancer diagnosis. Through cellular experiments, we successfully detected sialic acid overexpression on cancerous cells with high specificity. These fluorescent polymeric nanoparticles can be useful as a potential bioimaging probe for detecting diseased cells.
