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[Effects of D-methionine on the hematopoietic system injury in irradiated mice]
De-Guan Li1, Jun-Ling Zhang1, Hong-Ying Wu1
1Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College; Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China.
Abstract:
The aim of this study was to investigate the effects of D-methionine (D-met) on the hematopoietic system injury in irradiated mice. C57BL/6 mice were divided into control group, irradiated group, 300 mg/kg D-met plus irradiation group and 1000 mg/kg D-met plus irradiation group. The control mice received sham irradiation, and the mice in remainder groups were exposed to 7.5 Gy; 1,4,8 Gy and 1 Gy of (137)Cs γ-ray respectively, were used to detect the survival rate, survival rate of bone marrow cells, WBC and its differential counts as well the colony formation ability in irradiated mice, respectively. The D-met was intraperitoneally injected to mice at 30 min before irradiation. The results showed that 300 and 1000 mg/kd D-met did not obviously enhance the survival rate of mice exposed to 7.5 Gy; the 10(-2),10(-3),10(-4) mol/L D-met significantly increased the survival rate of bone marrow cells in mice exposed to 1,4,8 Gy; 300 and 1000 mg/kg D-met even so increased the WBC count of peripheral blood in mice exposed to 1 Gy, but there was no statistical difference as compared with irradiated alone mice, moreover 300 and 1000 mg/kg D-met could obviously promote the colony formation ability of bone marrow cells in irradiated mice, the CFU-GM count was higher than that in 1 Gy irradiated mice (P < 0.05). It is concluded that the D-met can effectively mitigate the marrow cell injury resulted from irradiation, enhance the survival rate of bone marrow cells in irradiated mice, promote the recovery of hematopoietic function from radiation injury in mice.
Insights
D-methionine (D-met) mitigates radiation-induced hematopoietic injury in mice. It enhances bone marrow cell survival and promotes hematopoietic function recovery after irradiation.
Area of Science:
- Radiation Biology
- Hematology
- Pharmacology
Context:
- Ionizing radiation exposure causes significant hematopoietic system damage.
- Radiation-induced hematopoietic injury impacts bone marrow cell survival and function.
- Investigating radioprotective agents is crucial for mitigating radiation effects.
Purpose:
- To evaluate the efficacy of D-methionine (D-met) in ameliorating radiation-induced hematopoietic injury in mice.
- To assess D-met's effects on survival rates, bone marrow cell viability, and hematopoietic progenitor cell function post-irradiation.
Summary:
- D-methionine administration did not significantly improve overall mouse survival after high-dose irradiation (7.5 Gy).
- However, D-met significantly increased bone marrow cell survival rates across various irradiation doses (1, 4, 8 Gy).
- D-met promoted colony-forming unit-granulocyte-macrophage (CFU-GM) counts, indicating enhanced hematopoietic progenitor cell recovery.
Impact:
- D-methionine demonstrates radioprotective potential by mitigating marrow cell injury.
- D-met enhances bone marrow cell survival and aids in the recovery of hematopoietic function following radiation exposure.
- These findings suggest D-met as a potential therapeutic agent for radiation-induced hematopoietic damage.
