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Related Experiment Video

Updated: Apr 30, 2026

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High-mobility group box-1 protein determination in postmortem samples.

Cristian Palmiere1, Marc Augsburger1, Patrice Mangin1

  • 1University Center of Legal Medicine, Rue du Bugnon 21, 1011 Lausanne, Switzerland.

Forensic Science International
|April 26, 2014
PubMed
Summary
This summary is machine-generated.

High-mobility group box-1 protein (HMGB1) increases significantly after death, limiting its use for identifying sepsis-related deaths during autopsy. Postmortem HMGB1 levels are difficult to reliably estimate, even with sample dilution.

Keywords:
Forensic pathologyHigh-mobility group box-1 proteinPostmortem biochemistrySepsis

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Area of Science:

  • Forensic Pathology
  • Biochemistry
  • Immunology

Background:

  • High-mobility group box-1 protein (HMGB1) is a nuclear protein implicated in inflammatory responses.
  • Assessing HMGB1's diagnostic utility in postmortem settings is crucial for determining causes of death, particularly sepsis.

Purpose of the Study:

  • To determine if high-mobility group box-1 protein (HMGB1) can be measured in autopsy biological fluids.
  • To evaluate HMGB1's diagnostic potential for identifying sepsis-related deaths.

Main Methods:

  • HMGB1 levels were measured in antemortem serum, postmortem serum, and pericardial fluid from sepsis and control cases.
  • Samples were collected within 6 hours postmortem, with full biological sample availability.

Main Results:

  • HMGB1 levels markedly increased after death in all measured biological fluids.
  • Concentrations exceeded calibration limits in undiluted postmortem serum and pericardial fluid for all cases.
  • Postmortem HMGB1 levels were difficult to reliably quantify, even with sample dilution.

Conclusions:

  • The diagnostic potential of HMGB1 for identifying sepsis-related deaths in postmortem samples is severely limited.
  • Postmortem release of HMGB1 into biological fluids hinders accurate estimation of antemortem levels.
  • Further research may be needed to explore alternative biomarkers or refined methodologies for postmortem sepsis diagnosis.