Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

3.1K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
3.1K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

8.3K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
8.3K
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

39.5K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
39.5K
The Mitotic Spindle02:27

The Mitotic Spindle

7.1K
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
7.1K
The Mitotic Spindle02:27

The Mitotic Spindle

4.9K
4.9K
Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

3.1K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chlamydial membrane vesicles deliver the beta barrel outer membrane protein OmpA to mitochondria to inhibit apoptosis.

PLoS pathogens·2026
Same author

Continuum architecture dynamics of vesicle tethering in exocytosis.

Cell·2026
Same author

Evaluating MINFLUX experimental performance in silico.

Nature communications·2025
Same author

Multi-step implementation of meiotic crossover patterning.

bioRxiv : the preprint server for biology·2025
Same author

In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy.

Nature communications·2025
Same author

Effects of base temperature, immersion medium, and EM grid material on devitrification thresholds in cryogenic optical super-resolution microscopy.

Journal of structural biology·2025

Related Experiment Video

Updated: Apr 30, 2026

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

1.9K

The yeast kinetochore - structural insights from optical microscopy.

Konstanty Cieśliński1, Jonas Ries1

  • 1European Molecular Biology Laboratory (EMBL), Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.

Current Opinion in Chemical Biology
|April 26, 2014
PubMed
Summary

The kinetochore complex ensures accurate chromosome segregation during cell division. Optical imaging reveals the positions and amounts of key proteins in the budding yeast kinetochore.

More Related Videos

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

11.2K
4D Microscopy of Yeast
12:00

4D Microscopy of Yeast

Published on: April 28, 2019

7.7K

Related Experiment Videos

Last Updated: Apr 30, 2026

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

1.9K
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

11.2K
4D Microscopy of Yeast
12:00

4D Microscopy of Yeast

Published on: April 28, 2019

7.7K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Eukaryotic chromosomes are captured by spindle microtubules via the kinetochore, a conserved protein complex.
  • The kinetochore is crucial for correct chromosome segregation during cell division.
  • Budding yeast (Saccharomyces cerevisiae) kinetochore serves as a model due to its simpler structure.

Purpose of the Study:

  • To review findings on the budding yeast kinetochore.
  • To highlight the relative positions and stoichiometry of major kinetochore components.

Main Methods:

  • Optical imaging techniques were employed.
  • Analysis focused on the budding yeast (Saccharomyces cerevisiae) model system.

Main Results:

  • Optical imaging revealed the spatial arrangement of major budding yeast kinetochore proteins.
  • The stoichiometry of these protein components was elucidated.

Conclusions:

  • Understanding budding yeast kinetochore structure provides insights into chromosome segregation mechanisms.
  • This model system facilitates the study of conserved kinetochore functions in eukaryotes.