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Updated: Apr 30, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
A roadmap to the isotopolog space of flavocoenzymes
Adelbert Bacher1, Boris Illarionov, Wolfgang Eisenreich
1Department of Chemistry, Organic Chemistry & Biochemistry, Technische Universität München, Lichtenbergstraße 4, 85748 Garching, Munich, Germany, adelbert.bacher@t-online.de.
Abstract:
Flavocoenzymes with selective or universal stable isotope labeling are important tools for the investigation of flavoproteins using a variety of spectroscopic methods. Numerous selectively labeled flavin isotopologs can be generated by the combined application of chemical synthesis and in vitro biotransformation using commercially available enzymes and/or recombinant riboflavin biosynthesis enzymes. Notably, the complex reaction sequences can be rapidly carried out using enzyme-assisted one-pot reaction strategies.
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