Engineering the stereochemistry of cephalosporin for specific detection of pathogenic carbapenemase-expressing bacteria

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Haibin Shi¹, Yunfeng Cheng, Kyung Hyun Lee

¹Departments of Radiology and Chemistry, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305 (USA).

Angewandte Chemie (International Ed. in English)

|April 26, 2014

Abstract:

Reported herein is the design of fluorogenic probes specific for carbapenem-resistant Enterobacteriaceae (CRE) and they were designed based on stereochemically modified cephalosporin having a 6,7-trans configuration. Through experiments using recombinant β-lactamase enzymes and live bacterial species, these probes demonstrate the potential for use in the specific detection of carbapenemases, including metallo-β-lactamases in active bacterial pathogens.

Keywords:

enzymes fluorescence fluorescent probes metalloenzymes stereochemistry

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