Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

7.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetically targeted photocatalytic organic dyes for spatiotemporally controlled organic synthesis in specific living cells.

Nature chemistry·2026
Same author

Tumor immune cell targeting chimeras reprogram tumor-associated macrophages.

Nature chemical biology·2026
Same author

Iterative Bump-and-Hole Engineering Creates a Bioorthogonal Reporter for <i>N</i>-Acetylglucosaminyltransferase I.

Journal of the American Chemical Society·2026
Same author

Carbon starvation of <i>Mycobacterium abscessus</i> induces a non-replicating state with extensive proteomic remodeling.

bioRxiv : the preprint server for biology·2026
Same author

Chemoproteomic Elucidation of β-Lactam Drug Targets in <i>Mycobacterium abscessus</i>.

ACS infectious diseases·2026
Same author

Mucin-binding protein shuttles enable delivery of brain-targeted therapeutics.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Apr 30, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.2K

An expanded set of fluorogenic sulfatase activity probes.

Elizabeth L Smith1, Carolyn R Bertozzi, Kimberly E Beatty

  • 1Departments of Chemistry and Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, B84 Hildebrand Hall, #1460, Berkeley, CA 94720 (USA).

Chembiochem : a European Journal of Chemical Biology
|April 26, 2014
PubMed
Summary

New fluorogenic probes detect sulfatases in mycobacterial lysates. These enzyme probes reveal species-specific patterns, aiding in the study of mycobacterial enzyme activities.

Keywords:
Mycobacterium tuberculosisfluorescent probeshydrolasesprotein imagingsulfatases

More Related Videos

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

12.3K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.2K

Related Experiment Videos

Last Updated: Apr 30, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.2K
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

12.3K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.2K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Fluorogenic probes are valuable tools for enzyme activity profiling in biological systems.
  • Sulfatases play crucial roles in various biological processes, and their detection is important for understanding microbial function.

Purpose of the Study:

  • To develop and validate novel fluorogenic probes for the detection of sulfatases.
  • To profile sulfatase activities in mycobacterial lysates.
  • To establish species-specific sulfatase banding patterns.

Main Methods:

  • Synthesis of two fluorogenic sulfatase probes: 3-O-methylfluorescein-sulfate and resorufin-sulfate.
  • Validation of probes using commercial sulfatase enzymes.
  • Application of probes in gel-resolved assays of mycobacterial lysates.

Main Results:

  • The developed fluorogenic probes successfully detected sulfatases in mycobacterial lysates.
  • Species-specific sulfatase banding patterns were observed in gel-resolved assays.
  • Probes demonstrated utility for enzyme activity profiling.

Conclusions:

  • 3-O-methylfluorescein-sulfate and resorufin-sulfate are effective tools for detecting sulfatases in mycobacteria.
  • These probes facilitate the differentiation of species-specific sulfatase profiles.
  • The probes serve as a foundation for developing more selective mycobacterial sulfatase detection methods.