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Gold labeling with wheat germ agglutinin and RNAse on osmicated tissue embedded in epoxy resin
1CSIRO, Division of Entomology, Canberra, Australia.
Abstract:
Tissue of an insect, Lucilia cuprina, fixed conventionally in buffered glutaraldehyde and osmium and embedded in epoxy resin (epon or epon/araldite), provided sections which could readily be labeled with RNAse/gold and wheat germ agglutinin (WGA)/gold. This method offers labeling of tissues with improved contrast and allows the retrospective application of RNAse and WGA labeling to conventionally prepared tissues, without recourse to oxidizing/etching agents.