Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deciphering the impact of zinc oxide nanopriming on growth and biochemical attributes of <i>Z. mays</i> under salinity stress.

Preparative biochemistry & biotechnology·2025
Same author

Fabrication of an electrochemical sensor based on molecularly imprinted polymer for the rapid detection of astaxanthin in environmental samples.

3 Biotech·2025
Same author

Encapsulation of melatonin in pH-responsive β-CD nanosponges for enhanced stability and optimized delivery in agriculture.

Plant physiology and biochemistry : PPB·2025
Same author

Enhancing cellulase performance through nanomaterials and MOFs: innovations and applications.

Preparative biochemistry & biotechnology·2025
Same author

Astaxanthin: a nature's versatile compound utilized for diverse applications and its therapeutic effects.

3 Biotech·2025
Same author

The role of nanoparticles in transforming plant genetic engineering: advancements, challenges and future prospects.

Functional & integrative genomics·2025

Related Experiment Video

Updated: Apr 30, 2026

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

12.9K

A new immobilization and sensing platform for nitrate quantification.

Veena Sachdeva1, Vinita Hooda1

  • 1Department of Botany, Faculty of Life Sciences, Maharshi Dayanand University, Rohtak 124001, India.

Talanta
|April 29, 2014
PubMed
Summary
This summary is machine-generated.

This study developed a novel biosensor by immobilizing nitrate reductase (NR) onto gold nanoparticles for accurate nitrate detection in groundwater. The biosensor demonstrated high sensitivity, stability, and reusability, offering a reliable alternative to traditional methods.

Keywords:
EpoxyGold nanoparticlesImmobilizationNitrate determinationNitrate reductase

More Related Videos

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

22.4K
Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

17.7K

Related Experiment Videos

Last Updated: Apr 30, 2026

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

12.9K
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

22.4K
Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

17.7K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Environmental Science

Background:

  • Nitrate contamination in groundwater poses significant environmental and health risks.
  • Accurate and sensitive detection methods for nitrate are crucial for water quality monitoring.
  • Enzyme immobilization on nanomaterials offers enhanced stability and activity for biosensing applications.

Purpose of the Study:

  • To develop a stable and sensitive biosensor for nitrate determination using immobilized nitrate reductase (NR) on epoxy-functionalized gold nanoparticles (epoxy/AuNPs).
  • To characterize the immobilized enzyme and optimize the biosensing conditions.
  • To evaluate the performance of the developed biosensor for real-world groundwater samples.

Main Methods:

  • Covalent coupling of Aspergillus niger nitrate reductase (NR) to epoxy/AuNPs.
  • Characterization using scanning electron microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR).
  • Optimization of pH, temperature, and substrate concentration for enzyme activity.
  • Determination of nitrate in groundwater and comparison with the Griess reaction method.

Main Results:

  • High conjugation yield (35.40±0.01 μg/cm(2)) and specific activity retention (93.90±0.85%) were achieved.
  • Optimized biosensor exhibited a low detection limit (0.05 mM) and wide linearity (0.1–10.0 mM).
  • Demonstrated excellent recovery (>95.0%), precision (CV < 3.125%), and strong correlation (R(2)=0.998) with the Griess method.
  • The immobilized NR showed good stability, retaining 50% activity after 16 reuses.

Conclusions:

  • The epoxy/AuNPs-immobilized nitrate reductase provides a highly stable, sensitive, and reusable platform for accurate nitrate detection in groundwater.
  • This novel biosensor offers a promising alternative to conventional methods for environmental water quality monitoring.
  • The developed method ensures reliable nitrate quantification with excellent performance metrics.