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Methicillin-susceptible Staphylococcus aureus CC398: first description in prosthetic joint infection and genetic

Guillaume G Aubin1, Didier Lepelletier1, Alain Reynaud1

  • 1EA3826, Thérapeutiques cliniques et expérimentales des infections - Faculté de Médecine, Nantes, France; Service de Bactériologie-Hygiène hospitalière, CHU de Nantes, France.

Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy
|April 29, 2014
PubMed

Insights

Methicillin-susceptible Staphylococcus aureus (MSSA) CC398 nasal carriage strains show genetic differences compared to infection strains. These differences in adhesins and immune evasion may explain their role in knee arthroplasty infections.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Methicillin-susceptible Staphylococcus aureus (MSSA) CC398 is an emerging pathogen.
  • Limited data exists on the genetic basis of CC398 MSSA infections.

Purpose of the Study:

  • To compare the genetic profiles of CC398 MSSA from nasal carriage and knee arthroplasty infections.
  • To identify genetic factors contributing to CC398 MSSA pathogenesis.

Main Methods:

  • DNA microarray analysis was used.
  • Strains were obtained from nasal carriage and knee arthroplasty infection sites.

Main Results:

  • CC398 MSSA from infection demonstrated acquisition of specific adhesins.
  • Enhanced iron capture systems were observed in infection strains.
  • Mechanisms for immune defense evasion were more prevalent in infection strains.

Conclusions:

  • Genetic modifications in CC398 MSSA strains are associated with infection.
  • Acquired virulence factors likely contribute to the pathogenesis of knee arthroplasty infections by CC398 MSSA.
Keywords:
DNA microarrayLivestock associated Staphylococcus aureus CC398Prosthetic-related infection

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