Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Space-Time Curvature and the General Theory of Relativity01:17

Space-Time Curvature and the General Theory of Relativity

4.6K
In 1905, Albert Einstein published his special theory of relativity. According to this theory, no matter in the universe can attain a speed greater than the speed of light in a vacuum, which thus serves as the speed limit of the universe.
This has been verified in many experiments. However, space and time are no longer absolute. Two observers moving relative to one another do not agree on the length of objects or the passage of time. The mechanics of objects based on Newton's laws of...
4.6K
P-N junction01:11

P-N junction

1.3K
A p-n junction is formed when p-type and n-type semiconductor materials are joined together. At the interface of the p-n junction, holes from the p-side and electrons from the n-side begin to diffuse into the opposite sides due to the concentration gradient. This diffusion of carriers leads to a region around the junction where there are no free charge carriers, known as the depletion region. The charge density within the depletion region for the n-side and p-side can be described by the...
1.3K
Space Trusses01:25

Space Trusses

1.3K
A space truss is a three-dimensional counterpart of a planar truss. These structures consist of members connected at their ends, often utilizing ball-and-socket joints to create a stable and versatile framework. The space truss is widely used in various construction projects due to its adaptability and capacity to withstand complex loads.
At the core of a space truss lies the fundamental unit known as the tetrahedron. This structure is composed of six members that form a three-dimensional shape...
1.3K
State Space Representation01:27

State Space Representation

610
The frequency-domain technique, commonly used in analyzing and designing feedback control systems, is effective for linear, time-invariant systems. However, it falls short when dealing with nonlinear, time-varying, and multiple-input multiple-output systems. The time-domain or state-space approach addresses these limitations by utilizing state variables to construct simultaneous, first-order differential equations, known as state equations, for an nth-order system.
Consider an RLC circuit, a...
610
GTPases and their Regulation02:14

GTPases and their Regulation

9.9K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.9K
Epigenetic Regulation01:46

Epigenetic Regulation

33.9K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synaptonemal complex SUMOylation is maintained by Nup60-dependent docking of Ulp1 at the nuclear periphery.

Cell reports·2026
Same author

Distinct and overlapping roles of MutLγ, Mus81-Mms4, and STR in meiotic Holliday junction processing.

Nature communications·2026
Same author

Critical appraisal of fairness metrics for artificial intelligence-based clinical prediction models: a scoping review.

The Lancet. Digital health·2026
Same author

Directional branch migration remodels the meiotic Holliday junction landscape.

bioRxiv : the preprint server for biology·2026
Same author

Evaluation of SOFA-2 Score Performance Across Demographic Subgroups: An External Validation Study Using MIMIC-IV.

medRxiv : the preprint server for health sciences·2026
Same author

Equiflow: An open-source software package for evaluating changes in cohort composition.

PLOS digital health·2026

Related Experiment Video

Updated: Feb 14, 2026

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
11:03

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Published on: December 8, 2021

4.5K

Holliday junction resolution: regulation in space and time.

Joao Matos1, Stephen C West1

  • 1London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK.

DNA Repair
|April 29, 2014
PubMed
Summary

Holliday junctions (HJs) are crucial for DNA repair and chromosome segregation. Cell-cycle kinases and phosphatases regulate HJ processing enzymes, ensuring proper DNA repair completion before cell division.

Keywords:
Cdc5Cell-cycleDNA repairEME1GEN1Mms4Mus81NucleasePLK1RecombinationResolvaseSlx1Slx4Yen1

More Related Videos

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

10.0K
Trajectory Data Analyses for Pedestrian Space-time Activity Study
16:14

Trajectory Data Analyses for Pedestrian Space-time Activity Study

Published on: February 25, 2013

14.2K

Related Experiment Videos

Last Updated: Feb 14, 2026

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
11:03

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Published on: December 8, 2021

4.5K
Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

10.0K
Trajectory Data Analyses for Pedestrian Space-time Activity Study
16:14

Trajectory Data Analyses for Pedestrian Space-time Activity Study

Published on: February 25, 2013

14.2K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Holliday junctions (HJs) are key intermediates in DNA recombinational repair.
  • Proper resolution of HJs is essential for accurate chromosome segregation during cell division.
  • Mechanisms regulating HJ processing pathway usage remain incompletely understood.

Purpose of the Study:

  • To elucidate the regulatory mechanisms controlling Holliday junction processing pathways.
  • To understand how cell-cycle control impacts HJ resolution during DNA repair.
  • To define the roles of specific nucleases in ensuring genome stability.

Main Methods:

  • Investigated the interplay between cell-cycle kinases/phosphatases and HJ-resolving nucleases.
  • Analyzed the spatial and temporal regulation of enzyme activities.
  • Examined the hierarchy of pathway usage in HJ metabolism.

Main Results:

  • A conserved network of cell-cycle kinases and phosphatases modulates HJ metabolism.
  • These regulators exert spatial and temporal control over structure-selective nucleases, including Mus81-Mms4 (yeast) and Yen1 (yeast).
  • This regulation establishes sequential activation of HJ processing enzymes, ensuring timely resolution.

Conclusions:

  • Mus81-Mms4/MUS81-EME1 and Yen1/GEN1 nucleases define a specialized class of enzymes.
  • These enzymes are critical for safeguarding DNA repair completion at the brink of chromosome segregation.
  • The findings reveal a sophisticated regulatory system ensuring genome integrity.