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Sequence-specific isotope effects on the cleavage of DNA by bleomycin
J W Kozarich1, L Worth, B L Frank
1Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742.
Science (New York, N.Y.)
|September 22, 1989
Abstract:
Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4'-hydrogen. A DNA fragment has been prepared that contains [4'-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology.