Related Experiment Video
Updated: Apr 30, 2026
07:50
Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis
Published on: December 8, 2023
1.3K
Antitubercular constituents from Premna odorata Blanco.
Stephen B Lirio1, Allan Patrick G Macabeo2, Erickson M Paragas3
1Graduate School, University of Santo Tomas, España, Manila 1015, Philippines.
Journal of Ethnopharmacology
|April 29, 2014
Summary
Premna odorata leaf extracts show potential for treating tuberculosis. A specific compound, 1-heneicosyl formate, demonstrated significant antimycobacterial activity, supporting its traditional medicinal use.
Area of Science:
- Ethnomedicine
- Natural Product Chemistry
- Microbiology
Background:
- Premna odorata Blanco (Lamiaceae) is traditionally used for tuberculosis treatment in the Philippines.
- This study investigates the antitubercular properties of Premna odorata leaf extracts.
Purpose of the Study:
- To evaluate the in vitro antimycobacterial activity of Premna odorata leaf extracts and fractions.
- To isolate and identify bioactive compounds responsible for the observed activity.
Main Methods:
- Extraction and solvent polarity-based fractionation of Premna odorata leaves.
- Silica gel chromatography for purification of active fractions.
- In vitro antimycobacterial assay against Mycobacterium tuberculosis H37Rv using Microplate Alamar Blue assay (MABA).
Main Results:
- Crude extracts showed weak activity (MIC≥128µg/mL).
- Fractions from the DCM sub-extract exhibited enhanced inhibitory potency (MIC=54 to 120µg/mL).
- 1-heneicosyl formate isolated from the active fraction showed significant activity (MIC=8µg/mL).
Conclusions:
- The study provides scientific validation for the traditional use of Premna odorata in tuberculosis treatment.
- 1-heneicosyl formate is a potential lead compound for antitubercular drug development.
Related Concept Videos
01:25Aromatic Compounds: Overview
11.8K
In general, the term ‘aromatic’ indicates a pleasant smell or fragrance from fresh flowers, freshly prepared coffee, etc. In the early history of organic chemistry, many benzene derivatives were isolated from the pleasant odor oils of the plants. For example, vanillin was isolated from the oil of vanilla, methyl salicylate from the oil of wintergreen, and cinnamaldehyde from the oil of cinnamon. They all had a pleasant odor; hence the name aromatic was given.
In 1825, Faraday...
In 1825, Faraday...
11.8K
01:15Anthelminthic Agents
103
Anthelmintic drugs differ significantly from antiparasitic therapies targeting protozoa, primarily due to differences in parasite biology. Whereas most protozoal treatments act on proliferating cells, anthelmintics are typically directed against mature, nonproliferative helminths. The therapeutic approach considers the helminth's reliance on neuromuscular coordination, glucose metabolism, and microtubular integrity for survival, reproduction, and localization within the host. Most anthelmintics...
103