Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Heterochromatin02:38

Heterochromatin

12.0K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
12.0K
Heterochromatin02:38

Heterochromatin

3.7K
3.7K
Euchromatin01:01

Euchromatin

6.7K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
6.7K
Euchromatin01:01

Euchromatin

2.7K
2.7K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.1K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.1K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted disruption of tumor vasculature via polyphenol nanoparticles to improve brain cancer treatment.

Cell reports. Physical science·2022
Same author

Synthesis and biological evaluation of 6-(pyrimidin-4-yl)-1H-pyrazolo[4,3-b]pyridine derivatives as novel dual FLT3/CDK4 inhibitors.

Bioorganic chemistry·2022
Same author

Timely and Appropriate Administration of Inhaled Argon Provides Better Outcomes for tMCAO Mice: A Controlled, Randomized, and Double-Blind Animal Study.

Neurocritical care·2022
Same author

Self-Assembled Epitaxial Ferroelectric Oxide Nanospring with Super-Scalability.

Advanced materials (Deerfield Beach, Fla.)·2022
Same author

Numerical Investigation of Heat Transfer Characteristics of scCO<sub>2</sub> Flowing in a Vertically-Upward Tube with High Mass Flux.

Entropy (Basel, Switzerland)·2022
Same author

TRIM21 suppresses CHK1 activation by preferentially targeting CLASPIN for K63-linked ubiquitination.

Nucleic acids research·2022

Related Experiment Video

Updated: Apr 30, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.4K

MTHFR promotes heterochromatin maintenance.

Bingtao Zhu1, Zhikai Xiahou1, Heyu Zhao1

  • 1Beijing Key Laboratory of DNA Damage Response and College of Life Science, Capital Normal University, Beijing 100048, China.

Biochemical and Biophysical Research Communications
|April 29, 2014
PubMed
Summary

Methylenetetrahydrofolate reductase (MTHFR) is phosphorylated by CDK1/Cyclin B1 during mitosis, affecting its activity. This MTHFR phosphorylation influences heterochromatin maintenance at centromeric regions.

Keywords:
CDK1/Cyclin B1HeterochromatinMTHFRMitosisPhosphorylation

More Related Videos

A Screening Method for Identification of Heterochromatin-Promoting Drugs Using Drosophila
06:22

A Screening Method for Identification of Heterochromatin-Promoting Drugs Using Drosophila

Published on: March 12, 2020

5.5K
Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands
10:13

Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands

Published on: August 21, 2021

3.3K

Related Experiment Videos

Last Updated: Apr 30, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.4K
A Screening Method for Identification of Heterochromatin-Promoting Drugs Using Drosophila
06:22

A Screening Method for Identification of Heterochromatin-Promoting Drugs Using Drosophila

Published on: March 12, 2020

5.5K
Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands
10:13

Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands

Published on: August 21, 2021

3.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Methylenetetrahydrofolate reductase (MTHFR) is crucial for folate metabolism and methylation.
  • MTHFR is known to be phosphorylated, with T34 identified as a key site.

Purpose of the Study:

  • To investigate the in vivo phosphorylation of MTHFR at T34.
  • To identify the kinase responsible for MTHFR T34 phosphorylation.
  • To elucidate the functional consequences of MTHFR phosphorylation on enzyme activity and heterochromatin.

Main Methods:

  • Generation of a phospho-specific antibody for T34-phosphorylated MTHFR.
  • In vivo phosphorylation analysis of MTHFR during the cell cycle.
  • Kinase assays using cyclin-dependent kinase 1 (CDK1)/Cyclin B1 complex.
  • Enzymatic activity assays of MTHFR immunoprecipitated from mitotic cells.
  • Analysis of heterochromatin markers (H3K9me3) and centromeric heterochromatin transcription following MTHFR expression inhibition.

Main Results:

  • MTHFR is phosphorylated at T34 in vivo, with peak levels during mitosis.
  • The CDK1/Cyclin B1 complex was identified as the kinase responsible for MTHFR T34 phosphorylation.
  • MTHFR enzymatic activity was reduced in mitotic cells.
  • Inhibition of MTHFR expression led to decreased H3K9me3 levels and increased transcription of centromeric heterochromatin markers.

Conclusions:

  • CDK1/Cyclin B1 phosphorylates MTHFR at T34.
  • MTHFR plays a significant role in maintaining heterochromatin structure, particularly in centromeric regions.