In cell scalaradial interactome profiling using a bio-orthogonal clickable probe
C Cassiano1, L Margarucci, R Esposito
1Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, Salerno, Italy. mcmonti@unisa.it casapullo@unisa.it.
Chemical Communications (Cambridge, England)
|April 29, 2014
Abstract:
A bio-orthogonal click-chemistry procedure was developed to allow the in cell interactome profiling of scalaradial, an anti-inflammatory marine natural product. The results were validated through the application of the classical in vitro chemical proteomics and several bio-physical methods; peroxiredoxins, 14-3-3 isoforms and proteasomes were recognized as main scalaradial targets.
