Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revisiting Strategies for Bacterial Enumeration: The Case for Viable but Nonculturable (VBNC) Cells in Supplement Products.

Probiotics and antimicrobial proteins·2026
Same author

Elexacaftor/Tezacaftor/Ivacaftor Therapy in a Child With Cystic Fibrosis and Type 1 Spinal Muscular Atrophy.

Pediatric pulmonology·2026
Same author

Extracellular vesicles from the follicular fluid of competent oocytes improve blastocyst yields when supplemented during simulated physiological oocyte maturation in cattle.

Theriogenology·2025
Same author

Transforming personalized weight forecasting: From the Personalized Metabolic Avatar to the Generalized Metabolic Avatar.

Computers in biology and medicine·2025
Same author

Liposomal Doxorubicin, Vinblastine and Dacarbazine Plus Consolidation Radiotherapy of Residual Nodal Masses for Frontline Treatment in Older Adults With Advanced Stage Classic Hodgkin Lymphoma: Improved Outcome in a Multi-Center Real-Life Study.

Hematological oncology·2024
Same author

Inflammation and physical activity in multiple sclerosis patients. A systematic review and meta-analysis.

Complementary therapies in medicine·2024

Related Experiment Video

Updated: Apr 30, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

7.9K

In cell scalaradial interactome profiling using a bio-orthogonal clickable probe.

C Cassiano1, L Margarucci, R Esposito

  • 1Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, Salerno, Italy. mcmonti@unisa.it casapullo@unisa.it.

Chemical Communications (Cambridge, England)
|April 29, 2014
PubMed
Summary

Researchers developed a bio-orthogonal click-chemistry method to study how scalaradial, an anti-inflammatory marine compound, interacts with proteins inside cells. Key cellular targets including peroxiredoxins and proteasomes were identified.

More Related Videos

Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

37.4K
In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

6.7K

Related Experiment Videos

Last Updated: Apr 30, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

7.9K
Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

37.4K
In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

6.7K

Area of Science:

  • Marine Natural Products
  • Chemical Biology
  • Proteomics

Background:

  • Scalaradial is an anti-inflammatory marine natural product with potential therapeutic applications.
  • Understanding its molecular targets is crucial for elucidating its mechanism of action.
  • Existing methods may not fully capture in-cell interactions.

Purpose of the Study:

  • To develop and apply a novel bio-orthogonal click-chemistry approach for in-cell interactome profiling of scalaradial.
  • To identify the primary protein targets of scalaradial within a cellular environment.
  • To validate findings using complementary chemical proteomics and biophysical techniques.

Main Methods:

  • Development of a bio-orthogonal click-chemistry strategy for labeling scalaradial interactions.
  • In-cell interactome profiling using the developed click-chemistry procedure.
  • Validation via in vitro chemical proteomics and biophysical assays.

Main Results:

  • Successfully profiled the in-cell interactome of scalaradial.
  • Identified peroxiredoxins, 14-3-3 isoforms, and proteasomes as major scalaradial targets.
  • Confirmed targets through orthogonal validation methods.

Conclusions:

  • The developed bio-orthogonal click-chemistry method is effective for in-cell target identification of natural products.
  • Scalaradial interacts with key cellular proteins involved in oxidative stress response and protein degradation.
  • This study provides a foundation for further investigation into scalaradial's anti-inflammatory mechanisms.