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Coffee induces autophagy in vivo
Federico Pietrocola1, Shoaib Ahmad Malik2, Guillermo Mariño3
1Equipe 11 labellisée par la Ligue Nationale Contre le Cancer; INSERM U1138; Centre de Recherche des Cordeliers; Paris, France; Metabolomics and Molecular Cell Biology Platforms; Gustave Roussy; Villejuif, France; Université de Paris Sud; Villejuif, France.
Cell Cycle (Georgetown, Tex.)
|April 29, 2014
Summary
Coffee consumption, even decaffeinated, rapidly triggers autophagy, a cellular recycling process, in mice. This suggests coffee
Area of Science:
- Cell Biology
- Metabolic Health
- Nutritional Science
Background:
- Chronic coffee consumption is linked to reduced metabolic diseases and mortality.
- Autophagy, a cellular degradation process, plays a role in metabolic health.
- The cellular mechanisms underlying coffee's health benefits are not fully understood.
Purpose of the Study:
- To investigate the effect of coffee consumption on autophagy in vivo.
- To determine if coffee consumption impacts key regulators of autophagy and protein acetylation.
Main Methods:
- Mice were administered natural and decaffeinated coffee.
- Autophagic flux was measured by analyzing LC3B lipidation and p62/SQSTM1 levels in liver, muscle, and heart.
- Mammalian target of rapamycin complex 1 (mTORC1) activity and protein acetylation were assessed.
Main Results:
- Coffee consumption rapidly increased autophagic flux in multiple organs within 1-4 hours.
- Coffee inhibited mTORC1 activity, indicated by reduced p70(S6K) phosphorylation.
- Global protein deacetylation was observed following coffee consumption.
- These effects were consistent for both natural and decaffeinated coffee.
Conclusions:
- Coffee consumption rapidly induces autophagy and reduces protein acetylation in mice.
- These cellular changes mimic those observed during nutrient deprivation.
- Polyphenols in coffee may promote health by stimulating autophagy.
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