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Related Experiment Videos

Anion and amine uptake and uncoupling in submitochondrial particles.

G F Azzone, H Gutweniger, E Viola

    European Journal of Biochemistry
    |February 2, 1976
    PubMed
    Summary

    Submitochondrial particles show unique uncoupling responses to lipophilic anions and amines, differing from chloroplasts. This energy dissipation is catalytic, not stoichiometric, involving charge cycling.

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    Area of Science:

    • Biochemistry
    • Mitochondrial Physiology

    Background:

    • Submitochondrial particles (SMPs) are crucial for studying oxidative phosphorylation.
    • Chloroplasts exhibit distinct responses to uncouplers compared to SMPs.
    • Understanding uncoupling mechanisms is key to comprehending energy transduction.

    Purpose of the Study:

    • To investigate the uncoupling effects of lipophilic anions and amines in SMPs.
    • To differentiate the mechanisms of uncoupling in SMPs from those in chloroplasts.
    • To elucidate the role of lipophilicity and charge cycling in energy dissipation.

    Main Methods:

    • Utilized submitochondrial particles and chloroplasts for comparative studies.
    • Assessed uncoupling effects using lipophilic anions (e.g., bromthymolblue) and amines.

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  • Measured ATP synthesis inhibition and anion/amine uptake.
  • Investigated pH-dependency and the influence of other ions (e.g., Cl-).

    • Main Results:

    • SMPs are not uncoupled by nigericin or NH4Cl, unlike chloroplasts.
    • Lipophilic anions cause pH-independent uncoupling in SMPs.
    • Amine uncoupling in SMPs is associated with lipophilicity and enhanced by lipophilic anions, distinct from chloroplasts.
    • Uncoupling appears to be a catalytic energy dissipation process.

    Conclusions:

    • The uncoupling mechanisms in SMPs differ significantly from chloroplasts.
    • Lipophilic anions and amines induce uncoupling in SMPs via catalytic energy dissipation.
    • Charge cycling following membrane perturbation is the likely molecular mechanism of uncoupling in SMPs.